Liquid chromatography (LC) uses liquid as mobile phase (eluent). It is an analytical method that separates a mixture of compounds based on their physical and chemical differences.
High performance liquid chromatography (HPLC) is a method that introduces the mobile phase under high-pressure conditions resulting in rapid and high-performance separations.
The various interactions between the analyte, stationary phase (packing material), and mobile phase are the key factors for the separation. A wide variety of separation modes can be achieved by using particular combinations of stationary and mobile phases.
YMC-Triart is a multi-layered material prepared using tightly controlled particle formation technology which has been adapted from micro-reactor technology. This recently developed production process results in exceptionally narrow particle and pore size distributions.
With YMC-Triart, challenging pH and high temperature conditions are no longer a limitation to the day-to-day work in laboratories. Most importantly, due to its unique particle composition, a balanced hydrophobicity and silanol activity are achieved which makes YMC-Triart a “First Choice” column in method development.
YMC-Triart phases show great chemical stability due to the newly developed hybrid-silica. Even under high pH or high temperature conditions, the lifetime of YMC-Triart phases is more than 10 times greater than conventional reversed phase columns
YMC Triart HPLC/uPLC/Capillary Column
- Effective for method screening with various chemistries. 7 different bonded phases in current product line up.
- Great chemical durability provided by hybrid particles. Wide usable pH range 1-12 for alkyl phases.
- Uniform particle size contributes to superior peak shapes for a wide range of compounds and in various conditions
- UHPLC compatible column with operating pressure up to 100MPa packed with 1.9um particle
- Available in highly durable semi-preparative and preparative columns
- Also Available in Capillary column formats: ID: 75µm, 100µm, 300µm, 500 µm for Nano-LC and Micro-LC.
YMC’s classical reversed phase C18 packing material, is renowned worldwide because of its unique performance and reproducibility. There are various endcapping options available for different selectivity.
YMC- Pack ODS series HPLC columns
- Conventional ODS columns
- Four different material characteristics for customers with different needs
- Currently in use worldwide
CHIRAL ART are HPLC column/packing materials with polysaccharide derivatives chiral selector. CHIRAL ART are suitable for the separation of a wide range of chiral compounds, cis-trans isomers, and geometric isomers. CHIRAL ART are available in HPLC columns and in preparative grades, in large (multi kg) quantities.
Chiral Art
- Column material with Polysaccharide Derivatives chiral selector
- Chiral Selectors
- Column material with Polysaccharide Derivatives chiral selector
- Chiral Selectors;
- Amylose tris(3,5-dimethylphenylcarbamate)
- Cellulose tris(3,5-dimethylphenylcarbamate)
- Cellulose tris(3,5-dichlorophenylcarbamate)
- Cellulose tris(4-methylbenzoate)
- Cellulosetris(3-chloro-4-methylphenylcarbamate)
The separation of geometric and positional isomers is a challenging task in reversed-phase chromatography. Subtle molecular differences have to be recognized and resolved by the particular stationary phase being used. Polymeric C30 HPLC stationary phases such as YMC Carotenoid are able to discriminate isomeric structures of long chain molecules.
Compared to classical C18 stationary phases, YMC Carotenoid C30 is much more hydrophobic. Even when pure organic eluents are applied, many sample solutes are retained. The YMC Carotenoid C30 stationary phase provides sufficient phase thickness to enhance interaction with long chain molecules; therefore, geometric and positional isomers of conjugated double-bonding systems are recognized and resolved. The C30 resolving power for isomers can be verified by the separation of carotenoids, which has been the subject of considerable research efforts in the past.
Carotenoids are found in a variety of natural sources including fruits and vegetables. In addition, carotenoids are considered as potential drugs for cancer intervention and/or prevention. Despite the complexity of carotenoid extracts and the minor shape differences between carotenoid isomers, the separation, identification, and quantitation of these compounds can be achieved using YMC Carotenoid C30 columns.
Carotenoid C30 HPLC Column
- Excellent separation for carotenoids, applications widely featured in journals and reviews.
- Useful for geometric isomers separation
- Very lipophilic
- Exceptional selectivity pattern
- Isomer recognition
- Polar carotenes
- Polar and nonpolar xanthophylls
- Steroids
- Retinols
- Fat-soluble vitamins
- Available in analytical and capillary column hardware
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