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JCSG Plus Screen now available!


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Applications

  • Primary, diverse reagent system crystallization screen for proteins, complexes, peptides, nucleic acids, & water soluble small molecules

Features

  • Data mined sparse matrix screen
  • Efficiently samples salts, polymers, organics & pH
  • PEG & Salt versus pH
  • PEG & Salt
  • Low ionic strength versus pH
  • High [polymer] with low [salt]
  • Tube or Deep Well block format

The JCSG Plus is a general sparse matrix screen. 67 core reagents are based upon a statistical analysis performed by Page1 using results of the Joint Center for Structural Genomics (JCSG), in which a large number (473) of proteins from Thermotoga maritima were each set up against 480 reagents from 12 commercially available screens.

40 of the resulting 67 reagents (60%) are Hampton Research crystallization screen reagents (Crystal Screen, Crystal Screen 2, Crystal Screen Cryo, PEG/Ion Screen, Grid Screen Ammonium Sulfate, Grid Screen PEG 6000, Grid Screen PEG/LiCl, and Grid Screen MPD). The core 67 reagents are complemented with solutions from Index², an expansion of the JCSG selected by Newman² to fill out the pH profile of the screen and to enhance the range of precipitants by including neutralized organic acids³ and Tacsimate4. Therefore JCSG Plus is composed of 96 reagents, 69 (72%) of which are based on Hampton Research screens. If one wishes to screen beyond the reagents offered in JCSG Plus one should consider PEG/Ion or PEGRx for polymer biased reagents or SaltRx for salt biased reagents

JCSG Plus contains 96 unique reagents, 10 ml each.

JCSG Plus HT contains 96 unique reagents in a single Deep Well block format.

Ready-to-use reagents are sterile filtered and formulated with ultra-pure Type 1 water, using the highest purity salts, polymers, organics and buffers. Individual reagents are also available.

References and Readings

1. Page et al (2003). Shotgun crystallization strategy for structural genomics: an optimized two-tiered crystallization screen against the Thermotoga maritima proteome. Acta Cryst. D59, 1028-1037.

2. Newman et al (2005). Towards rationalization of crystallization screening for small- to medium-sized academic laboratories: the PACT/JCSG+ strategy. Acta Cryst. D61, 1426-1431.

3. A comparison of salts for the crystallization of macromolecules. Alexander McPherson. Protein Science (2001), 10:418-422.

4. Protein and Nucleic Acid Crystallization. Methods, A Companion to Methods in Enzymology, Academic Press, Volume 1, Number 1, August 1990.

5. A comparison of salts for the crystallization of macromolecules. McPherson, A. Protein Science, 10:418-422, 2001.

6. Polymers as nucleants under high salt conditions. McPherson, A. Oral presentation at RAMC 2001, San Diego, CA. USA.

7. A novel approach to crystallizing proteins under oil. D’Arcy, A. et al. Journal of Crystal Growth, (1996) 168, 175-180.


Interested in the JCSG Plus or Hampton Research crystallization screen reagents? Our team is available for further discussion at +65 6744 6645 or This email address is being protected from spambots. You need JavaScript enabled to view it..
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